Making Ends Meet: Chemically Mediated Circularization of Recombinant Proteins
نویسندگان
چکیده
A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.
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عنوان ژورنال:
دوره 14 شماره
صفحات -
تاریخ انتشار 2013